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human crispr metabolic gene knockout library file targets  (Addgene inc)


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    Addgene inc human crispr metabolic gene knockout library file targets
    Analysis of the preparation of two libraries (A) Representative of electrophoresis of first PCR product of Liu Human <t>CRISPR</t> Knockout Library with titered genome DNA as template. The backbone of this library is LentiCrispr V2. The best PCR condition is 4 μg genome DNA per reaction. (B) Representative of electrophoresis of first PCR product of Human <t>CRISPR</t> <t>Metabolic</t> Gene Knockout Library with titered genome DNA as template. The backbone of this library is LentiCrispr V1. The best PCR condition is 3 μg genome DNA per reaction. (C) Representative of electrophoresis of first and second PCR product of Liu Human CRISPR Knockout Library. For the second step PCR, primer pairs “i5 index N502” and “i7 index N701” were used. (D) Representative of electrophoresis of first and second PCR product of Human CRISPR Metabolic Gene Knockout Library. For the second step PCR, primer pairs “i5 index N502” and “i7 index N701” were used. (E) Upper panel: Representative bioanalyzer electropherograms of human metabolic libraries using an Agilent DNA 1000 kit. Lower panel: Representative bioanalyzer gel images of the same human metabolic libraries.
    Human Crispr Metabolic Gene Knockout Library File Targets, supplied by Addgene inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human crispr metabolic gene knockout library file targets/product/Addgene inc
    Average 91 stars, based on 1 article reviews
    human crispr metabolic gene knockout library file targets - by Bioz Stars, 2026-05
    91/100 stars

    Images

    1) Product Images from "Protocol of CRISPR-Cas9 knockout screens for identifying ferroptosis regulators"

    Article Title: Protocol of CRISPR-Cas9 knockout screens for identifying ferroptosis regulators

    Journal: STAR Protocols

    doi: 10.1016/j.xpro.2023.102762

    Analysis of the preparation of two libraries (A) Representative of electrophoresis of first PCR product of Liu Human CRISPR Knockout Library with titered genome DNA as template. The backbone of this library is LentiCrispr V2. The best PCR condition is 4 μg genome DNA per reaction. (B) Representative of electrophoresis of first PCR product of Human CRISPR Metabolic Gene Knockout Library with titered genome DNA as template. The backbone of this library is LentiCrispr V1. The best PCR condition is 3 μg genome DNA per reaction. (C) Representative of electrophoresis of first and second PCR product of Liu Human CRISPR Knockout Library. For the second step PCR, primer pairs “i5 index N502” and “i7 index N701” were used. (D) Representative of electrophoresis of first and second PCR product of Human CRISPR Metabolic Gene Knockout Library. For the second step PCR, primer pairs “i5 index N502” and “i7 index N701” were used. (E) Upper panel: Representative bioanalyzer electropherograms of human metabolic libraries using an Agilent DNA 1000 kit. Lower panel: Representative bioanalyzer gel images of the same human metabolic libraries.
    Figure Legend Snippet: Analysis of the preparation of two libraries (A) Representative of electrophoresis of first PCR product of Liu Human CRISPR Knockout Library with titered genome DNA as template. The backbone of this library is LentiCrispr V2. The best PCR condition is 4 μg genome DNA per reaction. (B) Representative of electrophoresis of first PCR product of Human CRISPR Metabolic Gene Knockout Library with titered genome DNA as template. The backbone of this library is LentiCrispr V1. The best PCR condition is 3 μg genome DNA per reaction. (C) Representative of electrophoresis of first and second PCR product of Liu Human CRISPR Knockout Library. For the second step PCR, primer pairs “i5 index N502” and “i7 index N701” were used. (D) Representative of electrophoresis of first and second PCR product of Human CRISPR Metabolic Gene Knockout Library. For the second step PCR, primer pairs “i5 index N502” and “i7 index N701” were used. (E) Upper panel: Representative bioanalyzer electropherograms of human metabolic libraries using an Agilent DNA 1000 kit. Lower panel: Representative bioanalyzer gel images of the same human metabolic libraries.

    Techniques Used: Electrophoresis, CRISPR, Knock-Out, Gene Knockout


    Figure Legend Snippet:

    Techniques Used: Recombinant, Modification, Saline, Staining, Viability Assay, DNA Purification, Purification, Gel Extraction, Plasmid Preparation, CRISPR, Knock-Out, Gene Knockout, Software, Cell Culture



    Similar Products

    human crispr metabolic gene knockout library file targets  (Addgene inc)
    91
    Addgene inc human crispr metabolic gene knockout library file targets
    Analysis of the preparation of two libraries (A) Representative of electrophoresis of first PCR product of Liu Human <t>CRISPR</t> Knockout Library with titered genome DNA as template. The backbone of this library is LentiCrispr V2. The best PCR condition is 4 μg genome DNA per reaction. (B) Representative of electrophoresis of first PCR product of Human <t>CRISPR</t> <t>Metabolic</t> Gene Knockout Library with titered genome DNA as template. The backbone of this library is LentiCrispr V1. The best PCR condition is 3 μg genome DNA per reaction. (C) Representative of electrophoresis of first and second PCR product of Liu Human CRISPR Knockout Library. For the second step PCR, primer pairs “i5 index N502” and “i7 index N701” were used. (D) Representative of electrophoresis of first and second PCR product of Human CRISPR Metabolic Gene Knockout Library. For the second step PCR, primer pairs “i5 index N502” and “i7 index N701” were used. (E) Upper panel: Representative bioanalyzer electropherograms of human metabolic libraries using an Agilent DNA 1000 kit. Lower panel: Representative bioanalyzer gel images of the same human metabolic libraries.
    Human Crispr Metabolic Gene Knockout Library File Targets, supplied by Addgene inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human crispr metabolic gene knockout library file targets/product/Addgene inc
    Average 91 stars, based on 1 article reviews
    human crispr metabolic gene knockout library file targets - by Bioz Stars, 2026-05
    91/100 stars
    		  Buy from Supplier

    Image Search Results


    Analysis of the preparation of two libraries (A) Representative of electrophoresis of first PCR product of Liu Human CRISPR Knockout Library with titered genome DNA as template. The backbone of this library is LentiCrispr V2. The best PCR condition is 4 μg genome DNA per reaction. (B) Representative of electrophoresis of first PCR product of Human CRISPR Metabolic Gene Knockout Library with titered genome DNA as template. The backbone of this library is LentiCrispr V1. The best PCR condition is 3 μg genome DNA per reaction. (C) Representative of electrophoresis of first and second PCR product of Liu Human CRISPR Knockout Library. For the second step PCR, primer pairs “i5 index N502” and “i7 index N701” were used. (D) Representative of electrophoresis of first and second PCR product of Human CRISPR Metabolic Gene Knockout Library. For the second step PCR, primer pairs “i5 index N502” and “i7 index N701” were used. (E) Upper panel: Representative bioanalyzer electropherograms of human metabolic libraries using an Agilent DNA 1000 kit. Lower panel: Representative bioanalyzer gel images of the same human metabolic libraries.

    Journal: STAR Protocols

    Article Title: Protocol of CRISPR-Cas9 knockout screens for identifying ferroptosis regulators

    doi: 10.1016/j.xpro.2023.102762

    Figure Lengend Snippet: Analysis of the preparation of two libraries (A) Representative of electrophoresis of first PCR product of Liu Human CRISPR Knockout Library with titered genome DNA as template. The backbone of this library is LentiCrispr V2. The best PCR condition is 4 μg genome DNA per reaction. (B) Representative of electrophoresis of first PCR product of Human CRISPR Metabolic Gene Knockout Library with titered genome DNA as template. The backbone of this library is LentiCrispr V1. The best PCR condition is 3 μg genome DNA per reaction. (C) Representative of electrophoresis of first and second PCR product of Liu Human CRISPR Knockout Library. For the second step PCR, primer pairs “i5 index N502” and “i7 index N701” were used. (D) Representative of electrophoresis of first and second PCR product of Human CRISPR Metabolic Gene Knockout Library. For the second step PCR, primer pairs “i5 index N502” and “i7 index N701” were used. (E) Upper panel: Representative bioanalyzer electropherograms of human metabolic libraries using an Agilent DNA 1000 kit. Lower panel: Representative bioanalyzer gel images of the same human metabolic libraries.

    Article Snippet: Download the Human CRISPR Metabolic Gene Knockout Library file targets 2,981 human genes with 29,790 guide RNAs ( https://www.addgene.org/pooled-library/sabatini-human-crispr-metabolic-knockout/ ) as lentiCRISPR_v1_library.txt.

    Techniques: Electrophoresis, CRISPR, Knock-Out, Gene Knockout

    Journal: STAR Protocols

    Article Title: Protocol of CRISPR-Cas9 knockout screens for identifying ferroptosis regulators

    doi: 10.1016/j.xpro.2023.102762

    Figure Lengend Snippet:

    Article Snippet: Download the Human CRISPR Metabolic Gene Knockout Library file targets 2,981 human genes with 29,790 guide RNAs ( https://www.addgene.org/pooled-library/sabatini-human-crispr-metabolic-knockout/ ) as lentiCRISPR_v1_library.txt.

    Techniques: Recombinant, Modification, Saline, Staining, Viability Assay, DNA Purification, Purification, Gel Extraction, Plasmid Preparation, CRISPR, Knock-Out, Gene Knockout, Software, Cell Culture